Flow cytometry

Flow cytometry

The sample cell being analyzed will be distributed evenly and then will enter into a thin tube of sheath fluid by the pressurized isotonic fluid. The sheath solution will surround the area around a narrow lumen and the sample will flow from the middle of the sheath fluid like a central flow, with each cell moving after another. The flow speed may reach 10 meters per second. To identify surface markers, the sample and specific monoclonal antibodies will be put next to one of the marked fluorochromes. In case a reaction happens, the surface of the cell will have fluorescence effect, thereby becomes hot by absorbing laser and reflects a longer wavelength. Reflected light will the measured at different angles. Delicacy of the cell devision depends on the shape and intensity of the laser. The laser beam has a diameter of about 2 micrometres and will hit the cells after moving through narrow lenses. The mesuring of laser scarreing will depend of the cell size. Analysis of fluorescence light reflection after exposure to laser light at 90 degrees and lateral angles provide information about some of antigenic status characterizations and intracellular contents.

Leave a Reply

Your email address will not be published.